Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.

  title={Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.},
  author={Jingli Yang and Jaeseon Yi and Chuanping Yang and Cheng Hao Li},
  journal={Tree physiology},
  volume={33 6},
An Agrobacterium tumefaciens-mediated transformation method was developed for Salix matsudana Koidz. using mature seeds as starting material. Multiple shoots were induced directly from embryonic shoot apices of germinating seeds. Although thidiazuron, 6-benzylaminopurine and zeatin induced multiple shoot induction with high frequency, zeatin (4.5 μM) was more effective for elongation of shoots and roots. The binary vector pCAMBIA1303, which contained neomycin phosphotransferase as a selectable… CONTINUE READING
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