Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations.

@article{BoissonDernier2001AgrobacteriumRR,
  title={Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations.},
  author={Aur{\'e}lien Boisson-Dernier and Mireille Chabaud and Francesc Garcia and Guillaume B{\'e}card and Charles Rosenberg and David G Barker},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2001},
  volume={14 6},
  pages={695-700}
}
Medicago truncatula, a diploid autogamous legume, is currently being developed as a model plant for the study of root endosymbiotic associations, including nodulation and mycorrhizal colonization. An important requirement for such a plant is the possibility of rapidly introducing and analyzing chimeric gene constructs in root tissues. For this reason, we developed and optimized a convenient protocol for Agrobacterium rhizogenes-mediated transformation of M. truncatula. This unusual protocol… CONTINUE READING
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