Agrobacterium-mediated transformation of the genome-sequenced poplar clone, nisqually-1 (Populus trichocarpa)

  title={Agrobacterium-mediated transformation of the genome-sequenced poplar clone, nisqually-1 (Populus trichocarpa)},
  author={Caiping Ma and Steven H. Strauss and Richard Meilan},
  journal={Plant Molecular Biology Reporter},
The US Department of Energy recently released a 6.8X draft of the genome sequence for Nisqually-1, a genotype of black cottonwood (Populus trichocarpa). To improve its utility for functional genomics research, having an efficient means for transformation and regeneration is necessary. To examine several parameters known to affect the transformation rate, we cocultivated leaf disc and stem explants with a strain ofAgrobacterium tumefaciens harboring a binary plasmid vector containing genes for… CONTINUE READING

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