Agrobacterium-mediated transformation of the desiccation-tolerant plant Craterostigma plantagineum

@article{Furini1994AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of the desiccation-tolerant plant Craterostigma plantagineum},
  author={Antonella Furini and Csaba Koncz and Francesco Salamini and Dorothea Bartels},
  journal={Plant Cell Reports},
  year={1994},
  volume={14},
  pages={102-106}
}
An efficient procedure for Agrobacterium tumefaciens- mediated transformation of the desiccation-tolerant plant Craterostigma plantagineum has been developed. Leaf explants were inoculated with A. tumefaciens strain GV3101 carrying the gene for kanamycin- or hygromycin-resistance and the ßglucuronidase reporter gene. Parameters which affected the transformation efficiency were the age of the explant, the degree of wounding and the presence of an antioxidant in the medium. Under optimal… CONTINUE READING