Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.

@article{Ishizaki2008AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.},
  author={Kimitsune Ishizaki and Shota Chiyoda and Katsuyuki T. Yamato and Takayuki Kohchi},
  journal={Plant & cell physiology},
  year={2008},
  volume={49 7},
  pages={1084-91}
}
Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary… CONTINUE READING