Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method

  title={Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method},
  author={Xiuren Zhang and R. Henriques and S. Lin and Qi-Wen Niu and N. Chua},
  journal={Nature Protocols},
  • Xiuren Zhang, R. Henriques, +2 authors N. Chua
  • Published 2006
  • Biology, Medicine
  • Nature Protocols
  • Collective efforts of several laboratories in the past two decades have resulted in the development of various methods for Agrobacterium tumefaciens–mediated transformation of Arabidopsis thaliana. Among these, the floral dip method is the most facile protocol and widely used for producing transgenic Arabidopsis plants. In this method, transformation of female gametes is accomplished by simply dipping developing Arabidopsis inflorescences for a few seconds into a 5% sucrose solution containing… CONTINUE READING
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