Agrobacterium-mediated transformation of sorghum : factors that affect transformation efficiency

@inproceedings{Carvalho2004AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of sorghum : factors that affect transformation efficiency},
  author={Carlos Henrique Siqueira de Carvalho and Usha Barwale Zehr and Nilupa S. Gunaratna and Joseph A Anderson and Halina H. Kononowicz and Thomas K. Hodges and John D. Axtell},
  year={2004}
}
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our… CONTINUE READING

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