Agrobacterium-mediated transformation of maize (Zea mays) immature embryos.

@article{Lee2014AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of maize (Zea mays) immature embryos.},
  author={Hyeyoung Lee and Zhanyuan J. Zhang},
  journal={Methods in molecular biology},
  year={2014},
  volume={1099},
  pages={
          273-80
        }
}
Agrobacterium tumefaciens-mediated transformation is one of the most efficient and simple gene delivery systems for genetic improvement and biology studies in maize. This system has become more widely used by both public and private laboratories. However, transformation efficiencies vary greatly from laboratory to laboratory for the same genotype. Here, we illustrate our advanced Agrobacterium-mediated transformation method in Hi-II maize using simple binary vectors. The protocol utilizes… 
View on PubMed
plantsci.missouri.edu
8 Citations
Background Citations
2
Methods Citations
1

8 Citations

Citation Type

Citation Type

Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta
TLDR
A novel Agrobacterium-mediated anther transformation for Wucai in planta is developed, and in this procedure, the male germ line was the predominant target, and this optimized transformation system could provide a useful tool for both the improvement of cultivar qualities and investigation of functional genes in Wucae.
Genetic and Genomic Toolbox of Zea mays
TLDR
The basics of working with maize are outlined and its rich genetic toolbox is described, ensuring this classic model system will continue to be at the forefront of basic biological study in the post-genomic era.
Use of genome editing technologies for genetic improvement of crops of tropical origin
TLDR
Genome editing technologies, such as CRISPR, allows precise and specific modification of genetic information for the improvement of crops of tropical origin to produce varieties with resistance or tolerance to biotic and abiotic factors.
Molecular advances on agricultural crop improvement to meet current cultivating demands
TLDR
Marker-assisted selection related to hybrid performance has shown that genomic selection is a successful approach to address complex quantitative traits and to facilitate speeding up breeding programs in these crops in the future.
Site‐specific recombinase genome engineering toolkit in maize
TLDR
Through the bombardment of recombinase activated DsRed transient expression constructs, it is determined that all five recombinases are functional in maize plants and could be utilized for a variety of genetic engineering applications, including selectable marker removal, targeted transgene integration into predetermined locations, and gene stacking.
DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny
TLDR
DNA methylation in the promoter region leads to silencing of exogenous genes in transgenic progeny of birch, suggesting that transgene silencing in the progeny is mainly due to DNA methylation at cytosine residues.
Natural variation in a type-A response regulator confers maize chilling tolerance
TLDR
It is demonstrated that natural variation in the type-A Response Regulator 1 (Zm RR1) gene leads to differences in chilling tolerance among maize inbred lines, and Transcriptome analysis indicates that ZmRR1 positively regulates the expression of ZmDREB1 and Cellulose synthase (CesA) genes to enhance chilling tolerance.
Effect of lipo-chitooligosaccharide on early growth of C4 grass seedlings
TLDR
This study shows that LCO addition induces the expression of genes involved in root growth promotion in C4 grasses.

References

SHOWING 1-10 OF 22 REFERENCES
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
TLDR
Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried “super-binary” vectors that carried stable integration, expression, and inheritance of the transgenes.
Improvement of Agrobacterium-mediated transformation in Hi-II maize (Zea mays) using standard binary vectors
TLDR
The enhanced Agrobacterium-mediated transformation of immature zygotic embryos of maize Hi-II using standard binary vectors is reported, which will be advantageous for maize genetic engineering studies including transformation-based functional genomics.
Improved Agrobacterium-mediated transformation of three maize inbred lines using MS salts
TLDR
Success is reported in generating transgenic plants and progeny from three maize inbred lines using an Agrobacterium-mediated standard binary vector system to target maize immature embryos.
Successful Agrobacterium-Mediated Genetic Transformation of Maize Elite Inbred lines
TLDR
An efficient transformation system was developed for maize elite inbred lines using Agrobacterium-mediated gene transfer by identifying important factors that affected transformation efficiency and should facilitate the introduction of agronomically important genes into commercial genotypes.
High throughput genetic transformation mediated by Agrobacterium tumefaciens in maize
TLDR
A high throughput genetic transformation system in maize has been developed with Agrobacterium tumefaciens mediated T-DNA delivery and represents a highly reproducible and reliable system for genetic transformation of maize Hi-II.
RECOVERY OF TRANSGENIC EVENTS FROM TWO HIGHLY RECALCITRANT MAIZE (Zea mays L.) GENOTYPES USING AGROBACTERIUM-MEDIATED STANDARD-BINARY-VECTOR TRANSFORMATION
TLDR
The results suggest that transformation of highly recalcitrant maize genotypes is achievable, although it is subject to the limited genetic potential of their regeneration or transformation capaci- ties.
Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants
TLDR
The results show that a transient heat shock treatment of primary transgenic callus is sufficient for inducing cre and excising the cre and nptII genes, and genetic segregation and molecular analysis confirmed that marker gene removal is precise, complete and stable.
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
TLDR
A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens, and sequence analysis revealed that the boundaries of the T-DNA in transgenic Rice plants were essentially identical to those intransgenic dicotyledons.
Agrobacterium-mediated transformation of seedling-derived maize callus
TLDR
Seedling-derived callus of five genotypes was used for Agrobacterium-mediated transformation and two constructs containing the green fluorescence protein gene and genes for either neomycin phosphotransferase II or glyphosate selection were used in transformation experiments.
High-frequency plant regeneration through callus initiation from mature embryos of maize (Zea Mays L.)
TLDR
Using this efficient regeneration system, plantlets were regenerated from seven elite maize inbred lines and provided a solid basis for genetic transformation of maize.
...
1
2
3
...