Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass.

@article{Chai2003AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass.},
  author={Ming-liang Chai and Bing-liang Wang and Jae-Yeoul Kim and Jong-Min Lee and Doo-Hwan Kim},
  journal={Journal of Zhejiang University. Science},
  year={2003},
  volume={4 3},
  pages={346-51}
}
Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with Agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation… CONTINUE READING
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