Agrobacterium-mediated transformation of grapefruit with the wild-type and mutant RNA-dependent RNA polymerase genes of Citrus tristeza virus

Citrus paradisi Macfad. ‘Duncan’ was transformed with constructs coding for the wild-type and mutant RNAdependent RNA polymerase (RdRp) of Citrus tristeza virus (CTV) for exploring replicase-mediated pathogen-derived resistance (RM-PDR). Th e RdRp gene was amplifi ed from a CTV genome and used to generate the wild-type and 2 mutant RdRp constructs for plant… CONTINUE READING