Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration

  title={Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration},
  author={Magdalena Cervera and Jos{\'e} Pina and Jose Luis Valencia Ju{\'a}rez and Luis Navarro and Leandro Pe{\~n}a},
  journal={Plant Cell Reports},
The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. Osbeck×Poncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day… CONTINUE READING
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