Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination

@article{Mishiba2005AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination},
  author={Kei-ichiro Mishiba and Dong Poh Chin and Masahiro Mii},
  journal={Plant Cell Reports},
  year={2005},
  volume={24},
  pages={297-303}
}
A transformation procedure for phalaenopsis orchid established by using immature protocorms for Agrobacterium infection was aimed at the introduction of target genes into individuals with divergent genetic backgrounds. Protocorms obtained after 21 days of culture on liquid New Dogashima medium were inoculated with Agrobacterium strain EHA101(pIG121Hm) harboring both β-glucuronidase (GUS) and hygromycin resistance genes. Subculture of the protocorms on acetosyringone-containing medium 2 days… CONTINUE READING

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