Agrobacterium-mediated transformation of Lupinus mutabilis L. using shoot apical explants

@article{Babaolu2000AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of Lupinus mutabilis L. using shoot apical explants},
  author={Mehmet Babaoğlu and Matthew Sean McCabe and J. Brian Power and Michael R. Davey},
  journal={Acta Physiologiae Plantarum},
  year={2000},
  volume={22},
  pages={111-119}
}
A procedure for regenerating plants of Lupinus mutabilis from shoot apices, from which the leaf primordia and initial cell layer(s) of the apical meristem were removed, has been used to generate transgenic plants following Agrobacterium tumefaciens-mediated gene delivery. Transformation competent cells, from which buds developed, were located at the periphery of the apical meristem. Kanamycin resistant plants were obtained which expressed β-glucuronidase activity. Integration of the neomycin… CONTINUE READING

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