Agrobacterium-mediated transformation of European chestnut embryogenic cultures

  title={Agrobacterium-mediated transformation of European chestnut embryogenic cultures},
  author={Elena Corredoira and Davis Montenegro and M. Carmen San-Jos{\'e} and Ana Mar{\'i}a Vieitez and Antonio Mart{\'i}n Ballester},
  journal={Plant Cell Reports},
An innovative and efficient genetic transformation protocol for European chestnut is described in which embryogenic cultures are used as the target material. When somatic embryos at the globular or early-torpedo stages were cocultured for 4 days with Agrobacterium tumefaciens strain EHA105 harbouring the pUbiGUSINT plasmid containing marker genes, a transformation efficiency of 25% was recorded. Murashige and Skoog culture medium containing 150 mg/l of kanamycin was used as the selection medium… CONTINUE READING
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