Agrobacterium-mediated transformation of Eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance

@article{Matsunaga2011AgrobacteriummediatedTO,
  title={Agrobacterium-mediated transformation of Eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance},
  author={Etsuko Matsunaga and Kazuya Nanto and Masatoshi Oishi and Hiroyasu Ebinuma and Yoshihiko Morishita and Nozomu Sakurai and Hideyuki Suzuki and Daisuke Shibata and Teruhisa Shimada},
  journal={Plant Cell Reports},
  year={2011},
  volume={31},
  pages={225-235}
}
Eucalyptus globulus is one of the most economically important plantation hardwoods for paper making. However, its low transformation frequency has prevented genetic engineering of this species with useful genes. We found the hypocotyl section with a shoot apex has the highest regeneration ability among another hypocotyl sections, and have developed an efficient Agrobacterium-mediated transformation method using these materials. We then introduced a salt tolerance gene, namely a bacterial… CONTINUE READING
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