Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon

  title={Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon},
  author={John P. Vogel and David F Garvin and Oymon M. Leong and Daniel M. Hayden},
  journal={Plant Cell, Tissue and Organ Culture},
Brachypodium distachyon (Brachypodium) has been proposed as a model temperate grass because its physical, genetic, and genome attributes (small stature, simple growth requirements, small genome size, availability of diploid ecotypes, annual lifecycle and self fertility) are suitable for a model plant system. Two additional requirements that are necessary before Brachypodium can be widely accepted as a model system are an efficient transformation system and homogeneous inbred reference genotypes… 
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
The identification of a transformable inbred diploid line, Bd21-3, and the development of a transformation method with transformation efficiencies as high as 41% of co-cultivated calluses producing transgenic plants are described.
A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21
An improved procedure is provided for the facile and efficient Agrobacterium-mediated transformation of line Bd21, a novel model system for structural and functional genomics studies of temperate grasses because of its biological and genetic attributes.
Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21 with two binary vectors containing hygromycin resistance and GUS reporter genes
The genome sequence of the standard line Bd21 has been released and genomic resources have been developed, and a method for efficient Agrobacterium-mediated Brachypodium transformation is developed.
Agrobacterium-Mediated Transformation of Brachypodium distachyon.
A method for Agrobacterium-mediated transformation of the inbred B. distachyon lines Bd21 and Bd3-3 is described, which has been shown to be efficient and facile in several studies that resulted in the creation of over 22,000 T-DNA mutants.
A high-throughput Agrobacterium-mediated transformation system for the grass model species Brachypodium distachyon L.
In the ongoing process of developing Brachypodium distachyon as a model plant for temperate cereals and forage grasses, we have developed a high-throughput Agrobacterium-mediated transformation
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis.
A facile, efficient and rapid transformation system for Bd21 is described using Agrobacterium-mediated transformation of compact embryogenic calli derived from immature embryos to facilitate large-scale functional genomics research in this model system.
Agrobacterium-mediated transformation of Brachypodium distachyon.
An efficient procedure enabling the Agrobacterium-mediated transformation of a range of diploid and polyploid genotypes of Brachypodium is described, which made possible the mass production of Bd21T-DNA mutant plant lines.
Molecular, morphological, and cytological analysis of diverse Brachypodium distachyon inbred lines.
The diverse brachypodium lines developed in this study will allow experimental approaches dependent upon natural variation to be applied to this new model grass and help efforts to have a better understanding of complex large genomes.
Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics.
This work constructed 2 bacterial artificial chromosome libraries from an inbred diploid Brachypodium distachyon line, Bd21, using restriction enzymes HindIII and BamHI to develop resources for genome research in this emerging model species and obtained a first view of the sequence composition of the Brachyp sodium genome.
Optimizing callus induction and proliferation for Agrobacterium-mediated transformation of Brachypodium distachyon
Results showed that 2.5 mg l−1 2,4-D is an optimal concentration to use for both callus induction and proliferation, although 5.0 mg l+1 may also be used for callus proliferation, and results indicated that hygromycin is significantly more efficient than bialaphos when using the Agrobacterium-mediated transformation system.


A rapid and efficient transformation protocol for the grass Brachypodium distachyon
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for
Brachypodium distachyon. A new model system for functional genomics in grasses.
A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of "core pooid" genera that contain
Plant regeneration and micropropagation of Brachypodium distachyon
It was concluded that B. distachyon performed well in tissue culture and was suitable for further studies aimed at genetic transformation and its continued development as a model molecular genetic system.
Factors influencing Agrobacterium-mediated transformation of monocotyledonous species
Extension of transformation protocols to elite genotypes and to more readily available explants in agronomically important crop species will be the challenge of the future.
T-DNA insertional mutagenesis for functional genomics in rice.
  • J. Jeon, S. Lee, G. An
  • Biology
    The Plant journal : for cell and molecular biology
  • 2000
The large population of T-DNA-tagged lines will be useful for identifying insertional mutants in various genes and for discovering new genes in rice.
Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.
The development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development and suggests that the identification and cloning ofArabidopsis genes expressed in any developmental process is feasible using this system.
Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism
Mutants of Agrobacterium tumefaciens which affect virulence or the ability to catabolize octopine were isolated after Tn5-induced mutagenesis, and three mutants contained insertions in the T-deoxyribonucleic acid.
Linkage of RFLP Markers to an Aluminum Tolerance Gene in Wheat
The symbol Alt BH is proposed to designate the major gene for Al tolerance located on 4DL, which can aid the transfer of this gene into sensitive germplasms and help identify DNA markers linked to Al tolerance gene(s).
A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium
This DNA transformation competent genomic library should expedite gene isolation, by gene rescue, in multicel–lular organisms like Arabidopsis, as with the unicellular organisms Escherichia coli and Saccharomyces cerevisiae.
T‐DNA insertion mutagenesis in Arabidopsis: mutational spectrum
The wide spectrum of mutants observed suggests that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion.