Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene

@article{Chan1993AgrobacteriummediatedPO,
  title={Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene},
  author={Ming-Tsair Chan and Hsin-Hsiung Chang and Shin-Lon Ho and Wu-Fu Tong and Su-May Yu},
  journal={Plant Molecular Biology},
  year={1993},
  volume={22},
  pages={491-506}
}
We have successfully transferred and expressed a reporter gene driven by an α-amylase promoter in a japonica type of rice (Oryza sativa L. cv. Tainung 62) using the Agrobacterium-mediated gene transfer system. Immature rice embryos (10–12 days after anthesis) were infected with an Agrobacterium strain carrying a plasmid containing chimeric genes of β-glucuronidase (uidA) and neomycin phosphotransferase (nptII). Co-incubation of potato suspension culture (PSC) with the Agrobacterium inoculum… CONTINUE READING

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