Agrobacterium-mediated genetic transformation of pigeon pea [Cajanus cajan (L.) Millsp.] for resistance to legume pod borer Helicoverpa armigera

@article{Krishna2010AgrobacteriummediatedGT,
  title={Agrobacterium-mediated genetic transformation of pigeon pea [Cajanus cajan (L.) Millsp.] for resistance to legume pod borer Helicoverpa armigera},
  author={Gaurav Krishna and Pranhitha Reddy and Pramod Wasudeo Ramteke and P. Rambabu and Kailas Bhagawanrao Tawar and Parthasarathi Bhattacharya},
  journal={Journal of Crop Science and Biotechnology},
  year={2010},
  volume={14},
  pages={197-204}
}
Agrobacterium-mediated genetic transformation was performed using embryonic axes explants of pigeon pea. Both legume pod borer resistant gene (cry1Ac) and plant selectable marker neomycine phosphor transferase (nptII) genes under the constitutive expression of the cauliflower mosaic virus 35S promoter (CaMV35S) assembled in pPZP211 binary vector were used for the experiments. An optimum average of 44.61% successfully hardened dot blot Southern hybridization positive plants were obtained on co… CONTINUE READING
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