Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

@inproceedings{Ribas2010AgrobacteriummediatedGT,
  title={Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures},
  author={Alessandra Ferreira Ribas and Eveline D{\'e}champ and Antony Champion and Benoit Patrick Bertrand and M. H. Combes and Jean-Luc Verdeil and Fabienne Lapeyre and Philippe Lashermes and H Meyer Etienne},
  booktitle={BMC Plant Biology},
  year={2010}
}
BackgroundFollowing genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic… CONTINUE READING
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