Agrobacterium-mediated co-transformation of multiple genes in upland cotton

  title={Agrobacterium-mediated co-transformation of multiple genes in upland cotton},
  author={Fei-Fei Li and Shen-Jie Wu and Tian-Zi Chen and Jie Zhang and Hai-Hai Wang and W Z Guo and T Zhang},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
Two cotton genotypes, Simian 3 (SM 3) and WC, were co-transformed using a mixture of four Agrobacterium tumefaciens cultures of strain LBA4404, each carrying a plasmid harboring the following genes, Bt + sck (for Bacillus thuringenesis protein and modified Cowpea trypsin inhibitor), bar (for glufosinate), keratin, and fibroin. The frequency of callus induction, embryogenesis, and plant regeneration were notably different between the two genotypes. However, there were no differences between the… CONTINUE READING


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