Agrobacterium-mediated RMCE approach for gene replacement.

  title={Agrobacterium-mediated RMCE approach for gene replacement.},
  author={Kazuya Nanto and Keiko Yamada-Watanabe and Hiroyasu Ebinuma},
  journal={Plant biotechnology journal},
  volume={3 2},
We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a target cassette, and subsequent introduction of an exchange vector. The target cassette contains the npt and cod genes between oppositely orientated recognition sites (RS). The… CONTINUE READING