Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones

@article{Zhang1993AgrobacteriumCA,
  title={Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones},
  author={Lian-zhu Zhang and Patrick J. Murphy and Allen Kerr and Max E. Tate},
  journal={Nature},
  year={1993},
  volume={362},
  pages={446-448}
}
CONJUGAL opines secreted by crown gall tumours induce strains of Agrobacterium tumefaciens that are donors of Ti plasmids to produce a diffusible conjugation factor1. This enhances the conjugal transfer efficiency of the Ti plasmid in other strains of A. tumefaciens. This factor behaves as a secondary messenger, transmitting the environmental information to tra genes. Here we report the use of spectrometry to show that this factor is identical to synthetic N-(β-oxo-octan-l-oyl)-L-homoserine… 
Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria.
TLDR
Four acyl-HSL bioreporters were evaluated, based on tra of Agrobacterium tumefaciens, lux of Vibrio fischeri, las of Pseudomonas aeruginosa, and pigment production by Chromobacterium violaceum, for their ability to detect sets of 3-oxo acyl, 3-hydroxy, and alkanoyl- HSLs with chain lengths ranging from C4 to C12.
Induction and Loss of Ti Plasmid Conjugative Competence in Response to the Acyl-Homoserine Lactone Quorum-Sensing Signal
ABSTRACT Conjugative transfer of the Ti plasmids of Agrobacterium tumefaciens is controlled by a quorum-sensing system composed of TraR and its signal N-(3-oxo-octanoyl)-l-homoserine lactone. This
A new regulatory element modulates homoserine lactone-mediated autoinduction of Ti plasmid conjugal transfer
TLDR
It is suggested that traM functions to sequester TraR from the very small amounts of AAI produced under conditions when the agrocinopines are not present, suggesting that TraM is not a direct transcriptional regulator.
Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules.
TLDR
The ability to inactivate AHL (quorum quenching, QQ) might be useful in controlling virulence of many pathogenic bacteria.
The BlcC (AttM) Lactonase of Agrobacterium tumefaciens Does Not Quench the Quorum-Sensing System That Regulates Ti Plasmid Conjugative Transfer
TLDR
It is concluded that BlcC has no biologically significant effect on Ti plasmid transfer or the quorum-sensing system or its regulatory system.
Bacteriocin small of Rhizobium leguminosarum belongs to the class of N-acyl-L-homoserine lactone molecules, known as autoinducers and as quorum sensing co-transcription factors
Small bacteriocin was isolated from the culture broth of the gram-negative bacterium Rhizobium leguminosarum, which forms symbiotic nitrogen-fixing root nodules on a number of leguminous plants. The
Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography.
  • P. Shaw, G. Ping, S. Farrand
  • Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1997
TLDR
The assay can be used to screen cultures of bacteria for acyl-homoserine lactones, for quantifying the amounts of these molecules produced, and as an analytical and preparative aid in determining the structures of these signal molecules.
Hierarchical autoinduction in Ralstonia solanacearum: control of acyl-homoserine lactone production by a novel autoregulatory system responsive to 3-hydroxypalmitic acid methyl ester
TLDR
The acyl-HSL-dependent autoinduction system in R. solanacearum is part of a more complex autoregulatory hierarchy, since the transcriptional activity of PhcA is itself controlled by a novel autoreGulatory system that responds to 3-hydroxypalmitic acid methyl ester.
Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction
TLDR
It is shown that conjugation is regulated directly by a transcriptional activator, TraR, which requires conjugated factor as a coinducer to activate tra gene expression, a homologue of LuxR, the lux gene activator from Vibrio fischeri which also requires an endogenously synthesized diffusible coinduce.
Quorum-Dependent Mannopine-Inducible Conjugative Transfer of an Agrobacterium Opine-Catabolic Plasmid
TLDR
It is predicted that there exist wild-type agrobacterial strains harboring plasmids in which MOP induces a functional traR and, hence, conjugation, and that the anti-activator function of TraM is conserved.
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Molecular Genetics (Biology): An Overview | Sciencing Experiments in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ... Experimental Molecular Genetics | Biology | MIT