Agrobacterium Protocols

  title={Agrobacterium Protocols},
  author={Kan Wang},
  booktitle={Methods in Molecular Biology},
Studies in functional genomics and crop improvement programs often rely on the introduction and expression of transgenes in plants. There are two essential components required for in planta transgene expression, a plasmid vector on which the transgene sequence is carried and a delivery system capable of transferring the vector to the target cells. Agrobacterium -mediated plant transformation and the binary plasmid vector system is the preferred method of transgene delivery. The cloning… CONTINUE READING


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