Agrobacterium Protocols

@article{Wang2015AgrobacteriumP,
  title={Agrobacterium Protocols},
  author={Kan Wang},
  journal={Methods of Molecular Biology},
  year={2015},
  volume={1224}
}
  • Kan Wang
  • Published 2015
  • Biology
  • Methods of Molecular Biology
Within this chapter we outline an A. tumefaciens -mediated transformation method for B. rapa using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation effi ciencies are typically achieved in the region of 1 % (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection. 
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A protocol is presented for rapid genotype-independent transformation and regeneration of cotton (Gossypium spp.) from shoots isolated from germinating seedlings using a super-virulent strain of Agrobacterium tumefaciens and directly regenerated as shoots in vitro.
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