Agrobacterium Protocols

  title={Agrobacterium Protocols},
  author={Kan Wang},
  journal={Methods of Molecular Biology},
  • Kan Wang
  • Published 2015
  • Biology
  • Methods of Molecular Biology
Within this chapter we outline an A. tumefaciens -mediated transformation method for B. rapa using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation effi ciencies are typically achieved in the region of 1 % (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection. 
3 Citations
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It is proposed that effective transient transformation and efficient regeneration are the key for future utilization of genome editing technologies for improvement of fruit crops.
Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies
This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus, and explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation.
In vitro Effect of Replacement Mineral Salts of Trace Elements with P-containing Chelates to Improve Rooting of Cherry Rootstock (cv. VC-13)
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A protocol is presented for rapid genotype-independent transformation and regeneration of cotton (Gossypium spp.) from shoots isolated from germinating seedlings using a super-virulent strain of Agrobacterium tumefaciens and directly regenerated as shoots in vitro.
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Genetically engineered plants of commercial cotton varieties obtained by Agrobacterium–mediated transformation gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme.
Establishment of new axenic hairy root lines by inoculation with Agrobacterium rhizogenes
This work establishes forty original dicotyledonous hairy root lines with A. rhizogenes strain A4, which is known for approximately thirty plant species and spontaneously regenerated whole plants.
Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex
Evidence for integration of the transgene (GUS) was observed in two successive generations from the regenerants (T0), and the transformed plants appeared to have more than one copy of the T-DNA.
Somatic embryo initiation and germination in diploid cotton (Gossypium arboreum L.)
Improved results will help to pave the way for future genetic transformation and associated gene structure and function studies utilizing G. arboreum and can also be incorporated into regeneration protocols to improve the regeneration efficiency of other Gossypium species.
Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants
Cotton (Gossypium hirsutum L.) cotyledon tissues have been efficiently transformed and plants have been regenerated, facilitating transfer of genes of economic importance to cotton.
Transformation of Elite Cotton Cultivars via Particle Bombardment of Meristems
Molecular and genetic characterization of primary transformants and their progeny established that foreign genes were stably integrated and transmitted to progeny in a Mendelian fashion.
Sexually mature transgenic American chestnut trees via embryogenic suspension-based transformation
A high-throughput Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into the tree was established to produce transgenic events with an average efficiency of four independent transformation events per 50 mg of target tissue and minimal escapes.
An improved transformation protocol for studying gene expression in hairy roots of sugar beet (Beta vulgaris L.)
The molecular analysis and the nematode (Heterodera schachtii) resistance test in vitro demonstrated that the genomic structure and the expression of the Hs1pro-1-mediated nematodes resistance were well-maintained in all hairy root cultures even after repeated sub-culture.