Agrobacterium-Mediated Transformation of Barley Immature Embryos.

@article{Hinchliffe2019AgrobacteriumMediatedTO,
  title={Agrobacterium-Mediated Transformation of Barley Immature Embryos.},
  author={Alison Hinchliffe and Wendy A. Harwood},
  journal={Methods in molecular biology},
  year={2019},
  volume={1900},
  pages={
          115-126
        }
}
Barley transformation is an essential tool for a range of functional genomics studies as well as for future crop improvement. The demand for efficient crop transformation systems continues to grow, with new genome editing technologies adding to that demand. Here we describe an efficient and routine transformation protocol for the spring barley Golden Promise, based on Agrobacterium-mediated inoculation of immature embryos. This protocol has been widely used for overexpression and RNAi… 
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13 Citations
Background Citations
2
Methods Citations
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13 Citations

Citation Type

Citation Type

An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)
TLDR
A high-throughput, highly efficient and repeatable transformation system for wheat is reported and this system has been used successfully to introduce genes of interest, for RNAi, over-expression and for CRISPR–Cas9 based genome editing.
TRA1: a locus responsible for controlling Agrobacterium-mediated transformability in barley
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It is discovered that mutant M1460 had a transformation efficiencies similar to that of Golden Promise and proposed that one of the 225 genes in this region is TRA1, which is present in transformable lines only.
TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley
TLDR
It is discovered that mutant M1460 had a transformation efficiency similar to that of Golden Promise, and it is proposed that one of the 225 genes in this region is TRA1, present in transformable lines only.
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Investigation indicated that high copy numbers of repair template created by the replicon approach cause false positive PCR results which are indistinguishable at the sequence level to true GT events in junction PCR screens widely used in GT studies.
In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons
Advances in the use of RNA-guided Cas9-based genome editing in plants have been rapid over the last few years. A desirable application of genome editing is gene targeting (GT), as it allows a wide…
Genetic engineering and genome editing techniques in peanut plants
TLDR
Genome- editing technologies are recent and highly applied in plant research to improve genes associated with yield, disease resistance and drought resistance and genome editing and gene transformation technologies are extensively explored in this review.
Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance
TLDR
Surprisingly, it was found that the choice of promoter influenced the barley phenotype, not only increasing NUE and grain yield, but also improving nutrient uptake balance.
Mutations in Two Aphid-Regulated β-1,3-Glucanase Genes by CRISPR/Cas9 Do Not Increase Barley Resistance to Rhopalosiphum padi L
TLDR
This hypothesis that aphid-induced upregulation of callose-degrading β-1,3-glucanase genes in the host plant might counteract this negative effect on aphid performance is tested with barley mutants, which found no effect of these modified plant traits on barley resistance to R. padi.
The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley
TLDR
Assessment of the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK found all four genes were found to confer effective stem rust resistance and did not display significant adverse agronomic effects in the absence of disease.
The Effect of Endogenous Hormones, Total Antioxidant and Total Phenol Changes on Regeneration of Barley Cultivars
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Accumulated evidence suggests that for successful regeneration of recalcitrant cultivars, external treatments should be done in a way to reduce the inhibitory effects of internal factors.
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