Agreement Between Enzyme Linked Immunosorbent Assay with Rapid Plasma Reagin and Treponemapallidumhaem Agglutination Assay for Screening and diagnosis of syphilis at National Blood Bank Service in Addis Ababa, Ethiopia

@article{Afework2019AgreementBE,
  title={Agreement Between Enzyme Linked Immunosorbent Assay with Rapid Plasma Reagin and Treponemapallidumhaem Agglutination Assay for Screening and diagnosis of syphilis at National Blood Bank Service in Addis Ababa, Ethiopia},
  author={Ejigayehu Afework},
  journal={American Journal of Clinical Pathology},
  year={2019},
  volume={152}
}
A Thesis paper submitted to the Department of Medical Laboratory Sciences, School of Allied Health Science, College of Health Science, Addis Ababa University, in partial fulfillment of Master of Science Degree in Clinical Laboratory Sciences Hematology and Immunohematology. 

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TLDR
To evaluate the diagnostic performance of LIAISON Treponema Screen, a new automated chemiluminescence immunoassay (CLIA), in comparison with that of rapid plasma reagin (RPR) and the following currently used treponemal tests: hemagglutination test (TPHA), immunoenzymatic assay (EIA), and Western blot (WB). Expand
Comparative study of Treponemal and non-Treponemal test for screening of blood donated at a blood center
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Evaluating Enzyme-linked immunosorbent assay (ELISA) and Immunochromatographic assays in comparison with Treponema pallidum Haemagglutination Assay (TPHA) concluded that Omega Pathozyme can be considered as a suitable test for screening of syphilis in a blood center. Expand
Validation of the INNO-LIA Syphilis Kit as a Confirmatory Assay for Treponema pallidumAntibodies
TLDR
A novel multiparametric assay performed with the INNO-LIA Syphilis for the confirmation of syphilis antibodies in a set of 840 documented human serum samples provides reliable confirmatory diagnostic information that must currently be obtained by the performance and interpretation of results of a combination of serological assays. Expand
A comparison of the analytical level of agreement of nine treponemal assays for syphilis and possible implications for screening algorithms
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The performance of the treponemal serological assays was comparable while using medium and high-titre sera, but the varying performance on specimen dilutions suggests that there may be differences in sensitivity with low-tite sera that are more prevalent in primary and late syphilis cases. Expand
Comparative Evaluation of Nine Different Enzyme-Linked Immunosorbent Assays for Determination of Antibodies againstTreponema pallidum in Patients with Primary Syphilis
TLDR
Nine different enzyme-linked immunosorbent assays (ELISAs) with a sonicate or recombinant proteins of Treponema pallidum as antigen have been evaluated comparatively, and recommendations to use these tests as screening assays do need further data on specificity and reactivity in late stages of the disease. Expand
Profile of blood donors with serologic tests reactive for the presence of syphilis in São Paulo, Brazil
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The demographic characteristics and risk behaviors of blood donors with recent and past syphilis and their implications for blood bank testing and deferral strategies were determined. Expand
Rapid plasma reagin test: High false positivity or important marker of high risk behavior
TLDR
Sir, during the period from January to August 2013, a total of 9100 donors were screened for syphilis by RPR (Span Diagnostics Ltd.) at the blood bank. Expand
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TLDR
The results of this study highlight that the TPHA positivity was high (86.8%) in sera with VDRL titre less than 1:8, which means that for the diagnosis of syphilis, it is recommended that a confirmatory test such as TPHA should be performed on allSera with a reactive V DRL regardless of its titre. Expand
Importance of extending the use of polymerase chain reaction in the diagnosis of venereal syphilis in a blood transfusion center in Burkina Faso, West Africa
TLDR
Assessment of the effectiveness of PCR testing and serological methods in the diagnosis of Treponema pallidum subsp Pallidum among blood donors in Burkina Faso confirmed a low distribution of T. pallidums subsp pallidus in comparison to serology methods. Expand
Treponema pallidum hemagglutination assay seroreactivity among healthy Indian donors and its association with other transfusion transmitted diseases
TLDR
It is proposed that testing for syphilis by more sensitive and specific treponemal markers like TPHA rather than VDRL, rapid plasma reagin tests; as TPHA also has the added advantage of picking up all the high risk donors, whereas,VDRL picks up only currently infected donors. Expand
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