Agonists induce conformational changes in transmembrane domains III and VI of the beta2 adrenoceptor.

  title={Agonists induce conformational changes in transmembrane domains III and VI of the beta2 adrenoceptor.},
  author={Ulrik Gether and Shuangjun Lin and Pejman Ghanouni and Juan Antonio Ballesteros and Harel Weinstein and Brian K. Kobilka},
  journal={The EMBO journal},
  volume={16 22},
Agonist binding to G protein-coupled receptors is believed to promote a conformational change that leads to the formation of the active receptor state. However, the character of this conformational change which provides the important link between agonist binding and G protein coupling is not known. Here we report evidence that agonist binding to the beta2 adrenoceptor induces a conformational change around 125Cys in transmembrane domain (TM) III and around 285Cys in TM VI. A series of mutant… CONTINUE READING
76 Citations
62 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 76 extracted citations


Publications referenced by this paper.
Showing 1-10 of 62 references

Fluorescent labeling of 6746 purified beta 2adrenergic receptor : evidence for ligandspecific conformational changes

  • U. Gether, J. A. Ballesteros, R. Seifert, E. Sanders-Bush, H. Weinstein, B. K. Kobilka
  • J Biol . Chem .
  • 1995
Highly Influential
19 Excerpts

Integrated methods for the construction of three-dimensional models and computational probing of structure–function relations in G protein coupled receptors

  • J. A. Ballesteros, H. Weinstein
  • Methods Neurosci.,
  • 1995
Highly Influential
10 Excerpts

Similar Papers

Loading similar papers…