Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells.

  title={Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells.},
  author={Bernard Mouillac and M. Caron and H Bonin and Megan Dennis and Michel Bouvier},
  journal={The Journal of biological chemistry},
  volume={267 30},
The palmitoylation of the human beta 2-adrenergic receptor (beta 2-AR) was studied in recombinant baculovirus-infected insect Sf9 cells. At 48 h post-infection, a high level expression of an epitope-tagged beta 2-AR (10-25 pmol/mg protein) was detected by [125I]iodocyanopindolol ([125I]CYP) binding assays. The identity of the receptor was confirmed both by photoaffinity labeling and immunoblotting. The fusion receptor displayed typical beta 2-AR pharmacological properties and conferred a beta… CONTINUE READING
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