Agonist-induced phosphorylation of the kappa-opioid receptor.

@article{Appleyard1997AgonistinducedPO,
  title={Agonist-induced phosphorylation of the kappa-opioid receptor.},
  author={Suzanne M. Appleyard and Tucker A. Patterson and Wenzhen Jin and Charles Chavkin},
  journal={Journal of neurochemistry},
  year={1997},
  volume={69 6},
  pages={2405-12}
}
Antipeptide antibodies against the kappa-opioid receptor were used to test whether acute or chronic exposure to kappa agonists altered the phosphorylation state of the kappa-opioid receptor. Immunoprecipitation of the kappa receptor from guinea pig hippocampal slices preincubated in [32P]orthophosphoric acid revealed a basal phosphorylation of the kappa-opioid receptor. The amount of 32P incorporation into the receptor was increased following a 75-min treatment with the kappa agonist U50,488H… CONTINUE READING

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