Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.

@article{Tietz2003AgonistinducedCT,
  title={Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.},
  author={Pamela S. Tietz and Ra{\'u}l Alberto Marinelli and Xiqiang Chen and Bing Huang and Jonathan B. Cohn and Jolanta Kole and Mark A. McNiven and Seth L Alper and Nicholas F. LaRusso},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 22},
  pages={20413-9}
}
We previously proposed that ductal bile formation is regulated by secretin-responsive relocation of aquaporin 1 (AQP1), a water-selective channel protein, from an intracellular vesicular compartment to the apical membrane of cholangiocytes. In this study, we immunoisolated AQP1-containing vesicles from cholangiocytes prepared from rat liver; quantitative immunoblotting revealed enrichment in these vesicles of not only AQP1 but also cystic fibrosis transmembrane regulator (CFTR) and AE2, a Cl… CONTINUE READING