Agonist and blocking actions of choline and tetramethylammonium on human muscle acetylcholine receptors.

@article{Lape2009AgonistAB,
  title={Agonist and blocking actions of choline and tetramethylammonium on human muscle acetylcholine receptors.},
  author={Remigijus Lape and Paraskevi Krashia and David Colquhoun and Lucia G Sivilotti},
  journal={The Journal of physiology},
  year={2009},
  volume={587 Pt 21},
  pages={5045-72}
}
Choline has been used widely as an agonist for the investigation of gain-of-function mutants of the nicotinic acetylcholine receptor. It is useful because it behaves like a partial agonist. The efficacy of choline is difficult to measure because choline blocks the channel at concentrations about four times lower than those that activate it. We have fitted activation mechanisms to single-channel activity elicited from HEK-expressed human recombinant muscle nicotinic receptors by choline and by… CONTINUE READING