Aggregation of the intracellular domain of the type 1 tumor necrosis factor receptor defined by the two-hybrid system.

@article{Song1994AggregationOT,
  title={Aggregation of the intracellular domain of the type 1 tumor necrosis factor receptor defined by the two-hybrid system.},
  author={Ho Yeong Song and James D. Dunbar and David B. Donner},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 36},
  pages={22492-5}
}
The yeast-based two hybrid system has been used to determine whether oligomerization of the intracellular domain of the 55-kDa type 1 tumor necrosis factor (TNF) receptor may occur during TNF action. This assay depends upon reconstitution of the function of the GAL4 transcriptional activator through interaction of a protein fused to the GAL4 DNA binding domain with a protein fused to the transcriptional activation domain of GAL4. Fusion of the type 1 TNF receptor intracellular domain with the… CONTINUE READING