Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH.

@article{Sankoorikal2002AggregationOH,
  title={Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH.},
  author={B J Sankoorikal and Yong Lian Zhu and Michael E. Hodsdon and Elias Lolis and Priscilla S. Dannies},
  journal={Endocrinology},
  year={2002},
  volume={143 4},
  pages={1302-1309}
}
Aggregation of hormones is an important step in the formation of secretory granules that results in concentration of hormones. In transfected AtT20 cells, but not COS cells, Lubrol-insoluble aggregates of human prolactin (PRL) accumulated within 30 min after synthesis. Aggregation in AtT20 cells was reduced by incubation with 30 M chloroquine, which neutralizes intracellular compartments, and was slowed by incubation with diethyldithiocarbamate, which chelates Cu and Zn . H27A-PRL aggregated in… CONTINUE READING

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