Aggregation Stability of a Monoclonal Antibody During Downstream Processing

@article{Arosio2011AggregationSO,
  title={Aggregation Stability of a Monoclonal Antibody During Downstream Processing},
  author={Paolo Arosio and Giuliano Barolo and Thomas M{\"u}ller-Sp{\"a}th and Hua Y. Wu and Massimo Morbidelli},
  journal={Pharmaceutical Research},
  year={2011},
  volume={28},
  pages={1884-1894}
}
To study the effect of several operative parameters, particularly pH and salt concentration, on the stability and aggregation kinetics of IgG solutions under the conditions typically encountered in downstream processing. The time evolution of the aggregates is analyzed by a combination of dynamic light scattering, size exclusion chromatography (SEC) and field flow fractionation (FFF). Secondary structure changes are monitored by circular dichroism. For the given antibody, it is found that at pH… CONTINUE READING

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