Aggregation Mechanism of an IgG2 and two IgG1 Monoclonal Antibodies at low pH: From Oligomers to Larger Aggregates

@article{Arosio2012AggregationMO,
  title={Aggregation Mechanism of an IgG2 and two IgG1 Monoclonal Antibodies at low pH: From Oligomers to Larger Aggregates},
  author={Paolo Arosio and Simonetta Rima and Massimo Morbidelli},
  journal={Pharmaceutical Research},
  year={2012},
  volume={30},
  pages={641-654}
}
To identify the aggregation mechanism and the stability characteristics of three different monoclonal antibodies under acidic conditions. The aggregation kinetics is analyzed by a combination of light scattering, size exclusion chromatography and fluorescence techniques and the aggregation data are correlated to protein structure, hydrophobicity, charge and antibody subclass. In the investigated conditions, the antibody aggregation follows a mechanism consisting of two-steps: reversible monomer… CONTINUE READING