Aggregate formation of rCHO cells and its maintenance in repeated batch culture in the absence of cell adhesion materials.

Abstract

Aggregate formation of recombinant Chinese hamster ovary (rCHO) cells capable of producing granulocyte colony-stimulating factor (G-CSF), using medium lacking cell adhesion materials in a repeated batch culture, was examined together with cell growth, cell viability and G-CSF production. The rCHO culture was conducted in a rotary shaker and the medium was… (More)

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@article{Yamamoto2000AggregateFO, title={Aggregate formation of rCHO cells and its maintenance in repeated batch culture in the absence of cell adhesion materials.}, author={Shinya Yamamoto and H Matsuda and T. Takahashi and Xiaofang Xing and Y. Tanji and Hironori Unno}, journal={Journal of bioscience and bioengineering}, year={2000}, volume={89 6}, pages={534-8} }