Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies

  title={Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies},
  author={Alexandra Stolzing and Elena A. Jones and Dennis Mcgonagle and Andrew M. Scutt},
  journal={Mechanisms of Ageing and Development},

Comparison of the Donor Age-Dependent and In Vitro Culture-Dependent Mesenchymal Stem Cell Aging in Rat Model

It is suggested that ASCs expanded in vitro prior to their clinical use must be carefully screened for the possible aging effects resulting not only from donor age, but from the duration of their in vitro culture.

The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45lowCD271+ Phenotype

The data indicate that an individual's chronological age is not a reliable predictor of their MSC number or potency and suggest that old donor MSCs may not be inferior in regard to their multipotential functions.

Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells

There is an obvious interplay between donor age and cell passage that in the future must be accounted for when developing cell-based therapies for clinical use.

Age Dependent Changes of Biological Properties in the Multipotent Mesenchymal Stromal Bone Marrow Cells in FVB Mice

The obtained culture cells derived from adult and old mice according to phenotypic profile and capacity for directed multilineage differentiation meet minimum criteria for the MSCs.

The Effect of Age on Human Adipose-Derived Stem Cells

Infant-derived adipose-derived stem cells are morphologically spindle-shaped, with long telomeres, and exhibit enhanced angiogenic and osteogenic capabilities compared with older cells, and the authors posit that clinical applicability is conserved during the adult to elderly period.

Different facets of aging in human mesenchymal stem cells.

There is a growing perception that long-term culture has to be taken into account--especially for clinical applications, and the state of replicative senescence is poorly defined by the number of population doublings or even by thenumber of passages.

Age-related Changes in Bone Marrow Mesenchymal Stromal Cells

This review compares the various aspects of in vitro and in vivo MSC aging and suggests how the current knowledge on rejuvenating cultured MSCs could be applied to develop future strategies to target altered bone formation processes in OP and OA.

Characterization of bone‐marrow‐derived rat mesenchymal stem cells depending on donor age

The results indicate that aging individuals can be considered as candidates for autologous transplantation of bone‐marrow‐derived MSCs, which included phenotype, proliferating rate, osteogenic and adipogenic potential and secretory activity.

Age-specific changes of mesenchymal stem cells are paralleled by upregulation of CD106 expression as a response to an inflammatory environment.

Moderate levels of inflammatory stimuli are being interpreted by MSCs at a young age as instructive signals for osteoblastogenesis, whereas at old age, an inflammatory milieu may effectively suppress bone remodeling and repair by tissue-borne M SCs while uninterrupted adipogenic differentiation may lead to adipose upgrowth.

Changes of the Functional Capacity of Mesenchymal Stem Cells due to Aging or Age-Associated Disease – Implications for Clinical Applications and Donor Recruitment

A comprehensive overview of the recent findings with special attention to age-related changes of mesenchymal stem cell properties and the consequential impact on tissue regeneration and repair is presented.



Aging of mesenchymal stem cells

Skeletal progenitor cells and ageing human populations.

The maintenance of CFU-F number and alkaline phosphatase activity in these osteoarthritis patients may, in part, explain the inverse relationship observed for the preservation of bone mass between generalized osteOarthritis and primary osteoporosis.

Number and Proliferative Capacity of Osteogenic Stem Cells Are Maintained During Aging and in Patients with Osteoporosis

The study shows that the number and proliferative capacity of osteoprogenitor cells are maintained during aging and in patients with osteoporosis and that other mechanisms must be responsible for the defective osteoblast (OB) functions observed in these conditions.

Mesenchymal progenitor self-renewal deficiency leads to age-dependent osteoporosis in Sca-1/Ly-6A null mice

In vivo and in vitro analyses demonstrated that Sca-1 is required directly for the self-renewal of mesenchymal progenitors and indirectly for the regulation of osteoclast differentiation, suggesting that defective mesenchyal stem or progenitor cell self-Renewal may represent a previously uncharacterized mechanism of age-dependent osteoporosis in humans.

Study of Telomere Length Reveals Rapid Aging of Human Marrow Stromal Cells following In Vitro Expansion

The effect of in vitro expansion on the replicative capacity of MSCs is determined by correlating their rate of telomere loss during in vitro Expansion with their behavior in vivo, and it is reported that even protocols that involve minimal expansion induce a rapid aging of M SCs, with losses equivalent to about half their total replicative lifespan.

Differential regulation of smooth muscle markers in human bone marrow-derived mesenchymal stem cells

DMSO strongly downregulates the smooth muscle markers sm-calponin, short MLCK and sm-α-actin in human MSCs, indicating a transition from a smooth muscle-like phenotype to an undifferentiated state by an mTOR-dependent mechanism.

Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow.

Increased understanding of the induction of chondrogenic differentiation should lead to further progress in defining the mechanisms responsible for the generation of cartilaginous tissues, their maintenance, and their regeneration.

Age‐Related Osteogenic Potential of Mesenchymal Stromal Stem Cells from Human Vertebral Bone Marrow

It is demonstrated that the number of MSCs with osteogenic potential (CFU‐F/ALP+) decreases early during aging in humans and may be responsible for the age‐related reduction in osteoblast number.