Age-related changes in NMDA-induced [3H]acetylcholine release from brain slices of senescence-accelerated mouse.

Abstract

From the brain slices of normal mice (ddY strain, subcloned from dd strain in National Institute of Health in Japan), N-methyl-D-aspartic acid (NMDA) at 0.01-1 mM evoked [3H]acetylcholine (ACh) release in a concentration dependent manner. [3H]ACh release evoked by 1 mM NMDA was significantly inhibited by 2-amino-5-phosphonovaleric acid (APV), phencyclidine (PCP) and 5-methyl-10,11-dihydroxy-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK-801). The effects of NMDA were not seen in the Ca2+ free medium and were inhibited by physiological concentration (0.83 mM) of Mg2+. NMDA seems to cause ACh release from nerve terminals through the receptor-ion channel mediated mechanism in the mouse brain. Based upon these results, we determined the activity of a high K(+)- or NMDA-evoked [3H]ACh release using prone/8 strain of senescence-accelerated mouse (SAM-P/8) (a murine model of accelerated aging and memory dysfunction) and SAM-resistance/1 strain (SAM-R/1) (normal aging mice as the control) and these release activities were compared between both strains and during aging. [3H]ACh release evoked by 30 mM KCl was significantly lower than that of age-matched SAM-R/1 at 9 and 12 months. NMDA evoked the [3H]ACh release at 2, 6, 10 and 14 months in R/1 mice. In SAM-P/8 mice the activity of NMDA-evoked release was seen at 2 months, but markedly decreased afterwards. Nonsignificant difference was observed on the uptake of [3H]choline and on the spontaneous release of [3H]ACh between SAM-P/8 and SAM-R/1 strains, and during aging.(ABSTRACT TRUNCATED AT 250 WORDS)

Statistics

050100'98'00'02'04'06'08'10'12'14'16
Citations per Year

178 Citations

Semantic Scholar estimates that this publication has 178 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Zhao1992AgerelatedCI, title={Age-related changes in NMDA-induced [3H]acetylcholine release from brain slices of senescence-accelerated mouse.}, author={Xiao Hang Zhao and Yukihiko Kitamura and Y Nomura}, journal={International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience}, year={1992}, volume={10 2}, pages={121-9} }