Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin.

@article{Fu1995AgeEU,
  title={Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin.},
  author={Song-jun Fu and Chi Chen Fan and H W Song and Fang Wei},
  journal={Forensic science international},
  year={1995},
  volume={73 1},
  pages={35-40}
}
Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used. The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached. We determined the D/L… CONTINUE READING

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