Age Related Changes in 5‐methylcytosine Content in Human Peripheral Leukocytes and Placentas: an HPLC‐based Study

@article{Fuke2004AgeRC,
  title={Age Related Changes in 5‐methylcytosine Content in Human Peripheral Leukocytes and Placentas: an HPLC‐based Study},
  author={C. Fuke and M. Shimabukuro and A. Petronis and J. Sugimoto and T. Oda and K. Miura and T. Miyazaki and C. Ogura and Y. Okazaki and Y. Jinno},
  journal={Annals of Human Genetics},
  year={2004},
  volume={68}
}
The goal of the present study was to investigate inter‐individual and age‐dependent variation of global DNA methylation in human tissues. In this work, we examined 5‐methyldeoxycytidine (metC) content by HPLC in human peripheral blood leukocytes obtained from 76 healthy individuals of ages varying from 4 to 94 years (yr), and 39 human placentas from various gestational stages. The HPLC analysis revealed a significant variation of metC across individuals and is consistent with the previous… Expand
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