Agarose mold embedding of cultured cells for tissue microarrays.

  title={Agarose mold embedding of cultured cells for tissue microarrays.},
  author={Christopher A. Moskaluk and Mark H. Stoler},
  journal={Diagnostic molecular pathology : the American journal of surgical pathology, part B},
  volume={11 4},
There are several indications for the placement of samples of cultured cells in tissue microarrays (TMAs). To optimize this technique, three embedding procedures were compared: embedding of fixed cells pelleted by centrifugation, embedding of cells dispersed in an agarose matrix, and embedding of pelleted cells packed into the center of hollow agarose molds. TMAs were made from these preparations. The number of cells per tissue spot and the number of histologic sections that could be obtained… CONTINUE READING