Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan.

@article{Bhilocha2011AgaroseAP,
  title={Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan.},
  author={Shardul Bhilocha and Ripal Amin and Monika Pandya and Han Jing Yuan and Mihir Tank and Jaclyn C LoBello and Anastasia Shytuhina and Wenlan Wang and H G Wisniewski and Carol A. de la Motte and Mary K. Cowman},
  journal={Analytical biochemistry},
  year={2011},
  volume={417 1},
  pages={41-9}
}
Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5-500 kDa were investigated. For agarose-based systems, the suitability of different agarose types, agarose concentrations, and buffer systems was determined. Using chemoenzymatically synthesized HA standards of low polydispersity, the molecular mass range was determined for each gel composition over which the relationship between HA mobility… CONTINUE READING

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