Agarolytic bacterium Persicobacter sp. CCB‐QB2 exhibited a diauxic growth involving galactose utilization pathway

@article{Furusawa2017AgarolyticBP,
  title={Agarolytic bacterium Persicobacter sp. CCB‐QB2 exhibited a diauxic growth involving galactose utilization pathway},
  author={Go Furusawa and Nyok-Sean Lau and Appalasamy Suganthi and A. A. Amirul},
  journal={MicrobiologyOpen},
  year={2017},
  volume={6}
}
The agarolytic bacterium Persicobacter sp. CCB‐QB2 was isolated from seaweed (genus Ulva) collected from a coastal area of Malaysia. Here, we report a high‐quality draft genome sequence for QB2. The Rapid Annotation using Subsystem Technology (RAST) annotation server identified four β‐agarases (PdAgaA, PdAgaB, PdAgaC, and PdAgaD) as well as galK, galE, and phosphoglucomutase, which are related to the Leloir pathway. Interestingly, QB2 exhibited a diauxic growth in the presence of two kinds of… 
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Biochemical Characterization of Thermostable and Detergent-Tolerant β-Agarase, PdAgaC, from Persicobacter sp. CCB-QB2
TLDR
Although the major hydrolytic product was neoagarobiose (NA2), monomeric sugar was also detected by thin-layer chromatographic analysis, indicating the agarase may play an important role in the diauxic growth.
Agarase Production by Marine Pseudoalteromonas sp. MHS: Optimization, and Purification
TLDR
Biomass of the red alga Pterocladia capillacea proved to be a suitable substrate for agarase production using Pseudoalteromonas sp.
Crystal structure of a neoagarobiose-producing GH16 family β-agarase from Persicobacter sp. CCB-QB2
TLDR
The crystal structure of PdAgaC’s catalytic domain, which has one of the highest Vmax value at 2.9 × 103 U/mg, was determined in order to understand its unique mechanism.
Enhanced anti-oxidant activity of neoagarooligosaccharides produced by β-agarase derived from Aquimarina agarilytica NI125
TLDR
Results obtained suggest the potential application of the produced neoagarooligosaccharides anti-oxidants as promising additives in food and feed products.
Functional and Structural Studies of a Multidomain Alginate Lyase from Persicobacter sp. CCB-QB2
AlyQ from Persicobacter sp. CCB-QB2 is an alginate lyase with three domains — a carbohydrate-binding domain modestly resembling family 16 carbohydrate-binding module (CBM16), a family 32 CBM (CBM32)…
Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides
TLDR
Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community, and contribute to the understanding of bacteria–algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.
Mono-specific algal diets shape microbial networking in the gut of the sea urchin Tripneustes gratilla elatensis
TLDR
The dense and well-connected microbial network in the gut of Ulva-fed sea urchin Tripneustes gratilla elatensis, together with animal's rapid growth, may suggest that this alga was most nutritious among the experimental diets.
Climate Change Effects and Marine Renewable Energy Important Topics Targeted by the Journal of Marine Science
  • E. Rusu
  • Environmental Science
    Journal of Marine Science
  • 2022
 
Cloning and Characterization of Polysaccharide Degrading Genes from Selected Marine Bacteria

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OBJECTIVE To isolate and identify a versatile carbohydrate-degrading bacterium from marine environments, and characterize the extracellular agarase activity. METHODS The I2 staining method was…
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