Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

@article{Wiegand2008AgarAB,
  title={Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances},
  author={Irith Wiegand and Kai Hilpert and Robert E. W. Hancock},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={163-175}
}
The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. [] Key Method Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate.
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