Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction.

@article{Dirr1986AflatoxinBT,
  title={Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction.},
  author={Heini W. Dirr and Johannes C. Schabort},
  journal={Biochimica et biophysica acta},
  year={1986},
  volume={881 3},
  pages={
          383-90
        }
}
View on PubMed
doi.org
25 Citations
Highly Influential Citations
2
Background Citations
4
Results Citations
1

25 Citations

Characterization of the aflatoxin B1-binding site of rat albumin.
Biochemical and Molecular Action of Nutrients Carnitine Alters Binding of Aflatoxin to DNA and Proteins in Rat Hepatocytes and Cell-Free Systems
TLDR
Modulation of AFB1 binding to proteins and DNA by carnitine alters the carcinogenic and hepatotoxic potential of AFB1 and poses concerns about the human AFB1-exposure data based on the AFB 1-albumin adduct concentrations as a biomarker.
Structural dynamics studies on the binding of aflatoxin B1 to chicken egg albumin using spectroscopic techniques and molecular docking
TLDR
The overall study confirms conformational and structural alteration in the protein due to binding of AFB1 and suggests that the binding is enthalpy driven and van der Waals force and hydrogen bonds are stabilizing the AFB1-CEA complex.
Carnitine alters binding of aflatoxin to DNA and proteins in rat hepatocytes and cell-free systems.
TLDR
It is concluded that modulation of AFB(1) binding to proteins and DNA by carnitine alters the carcinogenic and hepatotoxic potential of AFB (1) and poses concerns about the human AFB-1)-exposure data based on the AFB( 1)-albumin adduct concentrations as a biomarker.
Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin
TLDR
It is demonstrated that aflatoxins form stable complexes with HSA as reflected by binding constants between 2.1 × 104 and 4.5 × 104 dm3/mol, and modeling studies and investigations with site markers suggest that Sudlow’s Site I of subdomain IIA is the high affinity binding site of a flatoxins on HSA.
Possible role of bovine serum albumin for the prevention of aflatoxin B1-absorption from the intestinal tract in young chicks.
TLDR
BSA may have AFB1-binding ability in the intestinal tract of young chicks and may also be excreted with AFB1, indicating that BSA must have protection from AFB1 as evidenced by the less pronounced changes in the liver of chicks inoculated with AFB 1 + BSA.
Evaluation of methods for quantitation of aflatoxin-albumin adducts and their application to human exposure assessment.
TLDR
Three complementary approaches to the quantitation of AF-albumin adducts are described and a combination of the hydrolysis ELISA for large scale screening followed by confirmatory analyses in positive samples by high-performance liquid chromatographic fluorescence is suggested as an optimum methodology.
Efficient Aflatoxin B1 Sequestration by Yeast Cell Wall Extract and Hydrated Sodium Calcium Aluminosilicate Evaluated Using a Multimodal In-Vitro and Ex-Vivo Methodology
TLDR
The results indicate that, by reducing the transmembrane transfer rate and reducing over 60% of the concentration of free AFB1, both products are able to significantly limit the bioavailability of AFB1.
The anticalmodulin effect of aflatoxin B1 on purified erythrocyte Ca2+-AtPase
TLDR
The genotoxic carcinogen aflatoxin B1 (AFB1) inhibited the calmodulin-stimulated membrane-bound (Ca2+Mg2+)-ATPase, suggesting that Ca2+-extrusion and other cal modulin-activated enzymes and processes may be slowed down during prolonged exposure to AFB1 because of its anticalmodulin effect.
The occurrence and detection of aflatoxin-macromolecular conjugates in humans.
TLDR
The results show that AFBi-lysine does occur in patients at King Edward VIII Hospital (KEH) and the highest level was detected in HCC patients followed by kwashiorkor patients, and strongly suggest that AF biO is able to covalently bind to lysine, histidine and arginine in albumin.
...
...

References

SHOWING 1-10 OF 43 REFERENCES
Fluorescence studies of the interactions of serum albumin and rat alpha1-fetoprotein with aflatoxin B1. Specificity and binding parameters.
Interactions of aflatoxin B1 and blood components of various species in vitro: interconversion of aflatoxin B1 and aflatoxicol in the blood.
Comparative study of the binding of coumarin anticoagulants and serum albumins.
Drug-binding properties of rat alpha 1-foetoprotein. Binding of warfarin, phenylbutazone, azapropazone, diazepam, digitoxin and cholic acid.
TLDR
The alpha 1-FP led to an increased affinity for warfarin, phenylbutazone and azapropazone without a change in the measured number of sites, indicating competition for binding at this site by (an) endogenous ligand(s).
A comparative study of some physico-chemical properties of human serum albumin samples from different sources--II. The characteristics of the N-B transition and the binding behaviour with regard to warfarin and diazepam.
Association of aflatoxin B1 with plasma components in vitro.
Interaction of aflatoxin B1 with DNA.
TLDR
Several lines of evidence suggest the possibility that aflatoxin exists in aqueous solution as aggregates, and the mechanism of the binding was discussed.
The effects of N-B transition of human serum albumin on the specific drug-binding sites.
Species difference in the binding of aflatoxin B1 to hepatic macromolecules.
Systemic excretion of benzo(a)pyrene in the control and microsomally induced rat: the influence of plasma lipoproteins and albumin as carrier molecules.
TLDR
It is inferred that metabolism of the plasma lipoprotein molecules determines, in part, the extent of benzo(a)pyrene excretion, which may represent a carcinogen pool that is slowly excreted.
...
...