Affinity purification of mitotic anaphase-promoting complex/cyclosome on p13Suc1.

@article{Hershko2005AffinityPO,
  title={Affinity purification of mitotic anaphase-promoting complex/cyclosome on p13Suc1.},
  author={Avram Hershko},
  journal={Methods in enzymology},
  year={2005},
  volume={398},
  pages={170-5}
}
A procedure is described for the affinity purification of the mitotic form of anaphase-promoting complex/cyclosome (APC/C) from HeLa cells. It is based on the binding of mitotically phosphorylated APC/C to the phosphate-binding site of p13(suc1), followed by specific elution with a phosphate-containing compound. The procedure is rapid, simple, and yields 50… CONTINUE READING