Affinity purification of eukaryotic 48S initiation complexes.

@article{Locker2006AffinityPO,
  title={Affinity purification of eukaryotic 48S initiation complexes.},
  author={Nicolas Locker and Laura E. Easton and Peter J. Lukavsky},
  journal={RNA},
  year={2006},
  volume={12 4},
  pages={
          683-90
        }
}
In vitro assembly of translation initiation complexes from higher eukaryotes requires purification of ribosomal subunits, eukaryotic initiation factors, and initiator tRNA from natural sources, and therefore yields only limited material for functional and structural studies. Here we describe a robust, affinity chromatography-based purification of eukaryotic 48S initiation complexes from rabbit reticulocyte lysate (RRL), which significantly reduces the number of individual purification steps… CONTINUE READING

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