Affinity purification of Hydra glutathione binding proteins

@article{Bellis1994AffinityPO,
  title={Affinity purification of Hydra glutathione binding proteins},
  author={Susan L. Bellis and David C. Laux and Dennis E. Rhoads},
  journal={FEBS Letters},
  year={1994},
  volume={354}
}
The association of glutathione (GSH) with putative external chemoreceptors elicits feeding behavior in Hydra. In the present study, solubilized membrane proteins were chromatographed on an affinity column of immobilized GSH in order to isolate GSH‐binding proteins that may represent the Hydra GSH chemoreceptor. The most abundant of the affinity‐purified proteins was a triplet of peptides ranging in molecular weight from 24.5–26 kDa. Antiserum generated against the 24.5–26 kDa triplet peptides… Expand
In situ potentiation of the glutathione-binding protein for the tentacle ball formation by a protease and efficient ingestion of prey in hydra.
  • K. Hanai
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
  • 1998
TLDR
Results indicate that the chemoreceptor potentiated in situ by a trypsin-like protease specifically evokes tentacle ball formation resulting in an efficient transfer of prey on the tentacle to the mouth. Expand
Different roles of GABA and glycine in the modulation of chemosensory responses in Hydra vulgaris (Cnidaria, Hydrozoa)
TLDR
GA appears to be the major inhibitory transmitter responsible for the regulation of the feeding response in polyps and molecular studies aimed at identifying GABA receptor subunits are in progress. Expand
Different roles of GABA and glycine in the modulation of chemosensory responses in Hydra vulgaris (Cnidaria, Hydrozoa)
Phylogenetic studies suggest that GABA and glycine receptors derive, as a result of divergent evolution, from a common ancestral protoreceptor originated in a unicellular organism. This raises theExpand
Arachidonic acid as an endogenous signal for the glutathione-induced feeding response in Hydra
TLDR
It is suggested that in Hydra AA may act as a second messenger following chemical stimulation, suggesting that the role of AA in the facilitation of the mouth-closing phase of the feeding response was supported. Expand
Measuring glutathione-induced feeding response in hydra.
TLDR
A simple method for the quantitation of the feeding response in hydra is described, in which the distance between the apical end of the tentacle and mouth of hydra is measured and the ratio of such distance before and after the addition of GSH is determined. Expand
Arachidonic acid and eicosanoids in Hydra: possible endogenous signals involved in chemoreception and modulation of the feeding behaviour.
TLDR
An in vivo study on the participation of AA and some of its metabolites in the GSH-induced feeding response in Hydra and shows that a population of GABAA-like receptors and/or GABA-gated Cl− channels are involved. Expand
EFFECTS OF GABAB LIGANDS ON THE GSH-INDUCED ELECTRICAL ACTIVITY OF THE HYPOSTOME IN HYDRA
TLDR
The results support the behavioral observations that GABA inhibits the cessation of the GSH-induced feeding response and indicates that GSH and GABA receptors are differentially distributed in the metazoan. Expand
Nitric Oxide Involvement in Hydra vulgaris Very Primitive Olfactory-Like System
TLDR
The results strongly implicate NO involvement in hydra very primitive feeding behavior, thus confirming its preservation throughout evolution. Expand
Glutathione and Signal Transduction in the Mammalian CNS
TLDR
The results demonstrate that GSH binds via its γ‐glutamyl moiety to ionotropic glutamate receptors, and support a reevaluation of the role of GSH in the nervous system in which GSH may be involved both directly and indirectly in synaptic transmission. Expand
Thiol-induced discharge of acontial nematocytes.
TLDR
Thiols are shown to be able to initiate the nematocyst discharge in isolated nematocytes and it is demonstrated for the first time that the modification of membrane thiols by selective agents may be a key event in the discharge of nematocyte. Expand
...
1
2
...

References

SHOWING 1-10 OF 20 REFERENCES
Partial characterization and detergent solubilization of the putative glutathione chemoreceptor from hydra.
TLDR
The fidelity of GSH binding in the solubilized preparation suggests that this preparation will be useful in further characterization of the putative glutathione chemoreceptor. Expand
Chemoreception in hydra: specific binding of glutathione to a membrane fraction.
TLDR
Data from saturation binding experiments indicates half maximal total specific binding occurs at 2 microM GSH which is similar to reported EC50 values from behavioral experiments, andCalcium is required for displaceable binding of GSH to the putative receptor. Expand
Chemoreception in Hydra vulgaris (attenuata): initial characterization of two distinct binding sites for L-glutamic acid.
TLDR
The identification of glutathione-insensitive glutamate binding is the first indication of a putative glutamate receptor, which may mediate an action independent of the glutathion-induced feeding response, and suggests that kainate and alpha-aminoadipate may be selective ligands for the glutATHione- insensitive and glutathION-sensitive glutamate binding sites, respectively. Expand
Glutathione-binding proteins identified by monoclonal antibodies which depress the behavioral response evoked by glutathione in Hydra.
TLDR
Molecular participating in the signaling pathway of R5 response with use of three mAbs suggested that J245 and J5 both acted on a component of the receptor-effector system which was not affected by J5/1, and 220 kDa proteins appear to be candidates for the receptor molecules mediating R5. Expand
The reaction of glutathione with amino acids and related compounds as catalyzed by gamma-glutamyl transpeptidase.
  • J. Revel, E. Ball
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 1959
TLDR
Methods which permit the quantitative estimation of the action of γ-glutamyl transpeptidase upon glutathione are described and attempts to account for all the main products formed in the reaction in both the presence and absence of the amino acid, arginine are presented. Expand
γ-Glutamyl transpeptidase in hydra
Abstract γ-Glutamyl transpeptidase (EC 2.3.2.2) activity is described in the coelenterate, Hydra attenuata , using the substrate γ-glutamyl-p-nitroanilide. The properties of the γ-glutamyl donorExpand
The apparent glutathione oxidase activity of gamma-glutamyl transpeptidase. Chemical mechanism.
TLDR
It is shown that covalent inhibitors of gamma-glutamyl transpeptidase abolish the oxidase activity of the purified enzyme and of crude homogenates of mouse and rat kidney. Expand
Activation of the Feeding Reflex in Hydra littoralis
  • H. Lenhoff
  • Biology, Medicine
  • The Journal of general physiology
  • 1961
TLDR
A simple and accurate quantitative assay of the glutathione-activated feeding reflex in Hydra is described and the present state of knowledge concerning the mechanism by which glutathion combines with and activates the glutATHione receptor to elicit the feeding reflex is summarized. Expand
Specific S-methylglutathione incorporation into a nematocyst-rich fraction of hydra.
TLDR
The results suggest that this is a new type of glutathione incorporation and could be explained by a type of receptor protein which accumulates glutATHione molecules. Expand
γ-Glutamyl transpeptidase in Hydralittoralis
TLDR
It is suggested that the cycle plays a role in the metabolism of glutathione in hydras and that γ-glutamyl transpeptidase may function in their digestive and absorptive processes and possibly also in the behavioral response to glutathion. Expand
...
1
2
...