Affinity purification of Hydra glutathione binding proteins

  title={Affinity purification of Hydra glutathione binding proteins},
  author={Susan L. Bellis and David C. Laux and Dennis E Rhoads},
  journal={FEBS Letters},

In situ potentiation of the glutathione-binding protein for the tentacle ball formation by a protease and efficient ingestion of prey in hydra.

  • K. Hanai
  • Biology
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
  • 1998

Different roles of GABA and glycine in the modulation of chemosensory responses in Hydra vulgaris (Cnidaria, Hydrozoa)

GA appears to be the major inhibitory transmitter responsible for the regulation of the feeding response in polyps and molecular studies aimed at identifying GABA receptor subunits are in progress.

Arachidonic acid as an endogenous signal for the glutathione-induced feeding response in Hydra

It is suggested that in Hydra AA may act as a second messenger following chemical stimulation, suggesting that the role of AA in the facilitation of the mouth-closing phase of the feeding response was supported.

Measuring glutathione-induced feeding response in hydra.

A simple method for the quantitation of the feeding response in hydra is described, in which the distance between the apical end of the tentacle and mouth of hydra is measured and the ratio of such distance before and after the addition of GSH is determined.

Arachidonic acid and eicosanoids in Hydra: possible endogenous signals involved in chemoreception and modulation of the feeding behaviour.

An in vivo study on the participation of AA and some of its metabolites in the GSH-induced feeding response in Hydra and shows that a population of GABAA-like receptors and/or GABA-gated Cl− channels are involved.


The results support the behavioral observations that GABA inhibits the cessation of the GSH-induced feeding response and indicates that GSH and GABA receptors are differentially distributed in the metazoan.

Nitric Oxide Involvement in Hydra vulgaris Very Primitive Olfactory-Like System

The results strongly implicate NO involvement in hydra very primitive feeding behavior, thus confirming its preservation throughout evolution.

Glutathione and Signal Transduction in the Mammalian CNS

The results demonstrate that GSH binds via its γ‐glutamyl moiety to ionotropic glutamate receptors, and support a reevaluation of the role of GSH in the nervous system in which GSH may be involved both directly and indirectly in synaptic transmission.

Thiol-induced discharge of acontial nematocytes.

Evidence that polycystins are involved in Hydra cnidocyte discharge

Experiments in this paper show that a PKD2 (polycystin 2) transient receptor potential (TRP) channel is expressed in hydra tentacles and bases, suggesting that polycystins play a direct or indirect role in cnidocyte discharge.



Partial characterization and detergent solubilization of the putative glutathione chemoreceptor from hydra.

The fidelity of GSH binding in the solubilized preparation suggests that this preparation will be useful in further characterization of the putative glutathione chemoreceptor.

Activation of the Feeding Reflex in Hydra littoralis

  • H. Lenhoff
  • Biology
    The Journal of general physiology
  • 1961
A simple and accurate quantitative assay of the glutathione-activated feeding reflex in Hydra is described and the present state of knowledge concerning the mechanism by which glutathion combines with and activates the glutATHione receptor to elicit the feeding reflex is summarized.