Affinity purification of Hydra glutathione binding proteins

  title={Affinity purification of Hydra glutathione binding proteins},
  author={S. L. Bellis and D. Laux and D. E. Rhoads},
  journal={FEBS Letters},
  • S. L. Bellis, D. Laux, D. E. Rhoads
  • Published 1994
  • Biology, Medicine
  • FEBS Letters
  • The association of glutathione (GSH) with putative external chemoreceptors elicits feeding behavior in Hydra. In the present study, solubilized membrane proteins were chromatographed on an affinity column of immobilized GSH in order to isolate GSH‐binding proteins that may represent the Hydra GSH chemoreceptor. The most abundant of the affinity‐purified proteins was a triplet of peptides ranging in molecular weight from 24.5–26 kDa. Antiserum generated against the 24.5–26 kDa triplet peptides… CONTINUE READING
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