Affinity proteomic approach for identification of an IgA-like protein in Litopenaeus vannamei and study on its agglutination characterization.

@article{Zhang2006AffinityPA,
  title={Affinity proteomic approach for identification of an IgA-like protein in Litopenaeus vannamei and study on its agglutination characterization.},
  author={Yueling Zhang and Sanying Wang and Anlong Xu and Jun Chen and Bokun Lin and Xuanxian Peng},
  journal={Journal of proteome research},
  year={2006},
  volume={5 4},
  pages={815-21}
}
An unknown protein reacted with anti-human IgA, namely, IgA-like protein, has been reported in shrimp, but information regarding its identification is not available. In the present study, an affinity proteomic strategy was applied to identify the IgA-like protein of shrimp Litopenaeus vannamei. The protein of 75 kDa was isolated and confirmed by affinity chromatography and Western blotting with goat anti-human IgA, respectively, and then identified as hemocyanin, a member of IgSF, by mass… CONTINUE READING

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