Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent--polymer aqueous two-phase system containing metal-chelating polymer.

Abstract

A system has been developed for selective partitioning of membrane proteins. For the first time, an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia coli, has been affinity partitioned in an aqueous two-phase system. The systems used were different detergent/polymer aqueous two-phase systems containing a metal-chelating polymer… (More)

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@article{Sivars2000AffinityPO, title={Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent--polymer aqueous two-phase system containing metal-chelating polymer.}, author={Ulf Sivars and J Abramson and Shigeru Iwata and Folke Tjerneld}, journal={Journal of chromatography. B, Biomedical sciences and applications}, year={2000}, volume={743 1-2}, pages={307-16} }